ASSOCIATION OF THE ESR1 GENE WITH REPRODUCTIVE TRAITS OF SOWS OF LARGE WHITE AND MIRGOROD BREEDS
The speciality of the modern methodology in breeding is using the molecular information, received during genome analysis. This methodology can significantly accelerate the improvement of productivity traits and it is particularly useful in relation to the traits with low coefficient of inheritance while classic methods are not effective enough. The reproductive traits are one of the most important in pig farming, estrogen receptor 1 gene (ESR1) is involved in their control. Meanwhile, the use of ESR1 locus polymorphism in the marker-assisted selection needs to determine the extent of its association with the reproductive traits of animals in those populations where it is planned to conduct such selection. Implementation of marker-assisted selection in Large White and Mirgorod breeds for improving the reproductive traits is an actual task, but a necessary step in this work is the associative analysis.
The purpose of the work is to research the association of polymorphisms of ESR1 locus with some reproductive traits of sows of Large White breed (ULW-1 and ULW-3 lines) and sows of Mirgorod breed.
Materials and methods of research. Experimental groups: 1) the sows of Large White breed, ULW-3 line, bred in "Bahmutskiy Agrarian Union" farm, Donetsk region; 2) the sows of Large White breed, ULW-1 line, bred in “Stepne” farm, Poltava region; 3) the sows of Mirgorod breed, bred in «Named after Dekabristy» farm, Poltava region. All the experimental animals were previously genotyped on RYR1 gene and had RYR1CC genotype. The animals were genotyped on estrogen receptor 1 locus with aid of PCR-RFLP analysis on PvuII-polymorphic restriction site in the third intron of the gene – DNA marker for estrogen receptor 1 gene. Associations between genotypes and the studied traits were calculated using ANOVA in Excel 2007.
Results. ULW-3 sows with ESR1BB genotype turned out to have 1.36 more piglets in a litter (analysing data from 2nd-4th farrows) comparing to animals with ESR1AA genotype. There is a tendency for bigger amount of newborn piglets in the heterozygotes animals than in sows with homozygous ESR1AA. A similar pattern appears in the 1st farrowing, the sows with ESR1BB and ESR1AB genotypes had the advantage in the total number of piglets at birth. In the experimental group of ULW-1 sows statistically proven patterns were not found, there was only a tendency to slight predominance of sows with ESR1BB and ESR1AB genotypes comparing to individuals with ESR1AA genotype. In the experimental group of Mirgorod sows there was a tendency to have most part of individuals with heterozygous genotype. Analysis of prolificacy of ULW-3 sows due to their genotype for the estrogen receptor 1 gene confirmed the superiority of ESR1BB and ESR1AB genotypes comparing to ESR1AA sows. According to 2nd-4th farrows, sows with ESR1BB and ESR1AB genotypes had the advantage in prolificacy comparing to ESR1AA sows by 1.15 and 0.53 piglets, respectively. According to the 1st farrowing difference between genotypes was absent. ESR1/PvuII-polymorphism do es not influence on prolificacy of ULW-1 sows. According to the 1st farrowing the trend towards a higher level of prolificacy of Mirgorod sows with ESR1AA genotype was found, while difference in 2nd-4th farrows between the groups was absent.
It was found that ESR1/PvuII-polymorphism impact on the total number of piglets at birth and prolificacy for ULW-3 sows is characterized by predominance of additive component with a little contribution of the dominant component, the similar trend is observed for ULW-1 sows. There is a complex nature of the impact of ESR1/PvuII-polymorphism on the reproductive traits of Mirgorod sows in the predominance of the dominant component.
Conclusions. The impact of polymorphism in estrogen receptor 1 gene on the total number of piglets in the litter after the birth and prolificacy in ULW-3 sows was detected. ULW-3 sows with ESR1BB genotype have 1.36 more piglets in a litter (analysing data from 2nd-4th farrows) and 1.15 more comparing to animals with ESR1AA genotype. ESR1/PvuII-polymorphism was not associated with total number of piglets in a litter and prolificacy in ULW-1 sows and Mirgorod sows. The counted parameters of additive-dominant model indicate that ESR1/PvuII polymorphism impact on the total number of piglets at birth and prolificacy for ULW-3 sows is characterized by predominance of additive component with a little contribution of the dominant component.
2. Aleksandrov, B. V., and E. E. Skorkina. 1992. Genetic and statistical characterization of breeding herds of large white breed. Bull. scientific papers Livestock Research Institute. 31:12–16 (in Ukrainian).
3. Chaykin, A. V. 2004. Efficiency of selection of gilts on reproductive qualities. Sankt-Peterburg, 108. – [Elektronnyiy resurs] – rezhim dostupa: http://www.dissercat.com/content/effektivnost-otbora-remontnykh-svinok-po-reproduktivnym-kachestvam#ixzz3zHc2PwT2 (in Ukrainian).
4. Rothschild, M. F., C. Jacobson, D. A. Vaske, C. K. Tuggle, L. Wang, T. Short, G. Eckardt, S. Sasaki, A. Vincent, D. G. McLaren, O. Southwood, H. van der Steen, A. Mileham, and G. Plastow. 1996. The oestrogen receptor locus is associated with a major gene influencing litter size in pigs. Proc. Natl. Acad. Sci. USA. 93: 201–205.
5. Isler, B. J., R. V. Irvin, S. M. Neal, S. J. Moeller, and M. E. Davis. 2002. Examination of the Relationship Between the Estrogen Receptor Gene and Reproductive Tract Components in Swine. Journal of Animal Science. 80(9):2334–2339.
6. Chen, K.F., LS Huang, N Li, Q Zhang, M Luo, and CX Wu. 2000. The genetic effect of estrogen receptor (ESR) on litter size traits in pig. J Genet Genomics. 27(10):853-857.
7. Van Rens, B.T.T.M., Hazeleger W., and van der Lende T. 2000. Periovulatory hormone profiles and components of litter size in gilts with different oestrogen receptor (ESR) genotypes. Theriogenology. 53:1375–1387.
8. Horogh, G., Zsolnai, I. Komlosi, A. Ny??ri, I. Anton, and L. Fes?us. 2005. Oestrogen receptor genotypes and litter size in Hungarian Large White pigs. J. Anim. Breed. Genet. 122:56–61.
9. Kostenko, S.O., M.V. Dragulyan, and O.V. Sidorenko. 2013. Osoblyvosti polimorfizmu heniv ESR, NCOA1, PRLR, FSHR u svyney riznykh porid - Features gene polymorphism ESR, NCOA1, PRLR, FSHR in pigs of different breeds. Manufacturing and processing of livestock products. 9:23-29 (in Ukrainian).
10. Balatskiy, V.N., A.M. Saenko, and L.P. Grishina. 2010. Polimorfizm lokusa receptora jestrogena 1 v populjacijah svinej raznyh genotipov i ego associacija s reproduktivnymi priznakami svinomatok krupnoj beloj porody – Polymorphism locus estrogen receptor 1 in populations of pigs of different genotypes and its association with reproductive traits of sows of large white breed. Cytology and Genetics. 46(4): 48–54 (in Ukrainian).
11. Gibson, J. P., Z. H. Jiang, J.A.B. Robinson, A. L Archibald., and C. S. Haley. 2002. No detectable association of the ESR Pvu II mutation with sow productivity in a Meishan x Large White F2 population. Animal Genetics. 33:448–450.
12. Alfonso, L. 2005. Use of meta-analysis to combine candidate gene association studies: application to study the relationship between the ESR PvuII polymorphism and sow litter size. Genet. Sel. Evol. 37:417– 435.
13. Dall'Olio, S., L. Fontanesi, L. Tognazzi, L. Buttazzoni M. Maurizio Gallo, and V. Russo. 2011. ESR1 and ESR2 gene markers are not associated with number of piglets born alive in Italian Large White sows. Italian Journal of Animal Science. 10:185–188.
14. Walsh, P. S., D. A. Metzger, and R. Higuch. 1991. Chelex 100 as a Medium for Extraction of DNA for PCR-Based Typing from Forensic Material. BioTechniques. 10:506–509.
15. Domashova, L. O. 2013. Asotsiatsiya vidtvoryuval'nykh yakostey svynomatok velykoyi biloyi porody z yikh henotypom po henu estrohenovoho retseptora – Association reproductive qualities of sows of large white breed of genotype on their estrogen receptor gene (ESR). Modern problems of selection and breeding animal hygiene. 2 (72):84–89 (in Ukrainian).
16. Balatskiy, V. N., A. M. Saenko, R. N. Pina, T. V. Buslik, and E.S. Gibolenko. 2015. Geneticheskaja differenciacija porod svinej po desjati lokusam kolichestvennyh priznakov – Genetic differentiation of pig breeds ten quantitative trait loci. Cytology and Genetics. 5:26–37 (in Ukrainian).
17. Santana, B. A. A., F. H. Biase, R. C. Antunes, M. Borges, M. Machaim Franco, and L. R. Goulart. 2006. Association of the estrogen receptor gene PvuII restriction polymorphism with expected progeny differences for reproductive and performance traits in swine herds in Brazil Genet. Mol. Biol. 29:273–277.
18. Van Rens, B. T., P. N. de Groot, and T. van der Lende. 2002. The effect of estrogen genotype on litter size and placental traits at term in F2 crossbred gilts. Theriogenol. 57:1635–1649.
This work is licensed under a Creative Commons Attribution 4.0 International License.