GROWTH AND DEVELOPMENT OF PIGS WITH DIFFERENT GENOTYPES OF SLC11A1 AND FUT1 DNA MARKER
The article highlights the results of research of the growth and development characteristics of pigs with different genotypes of DNA markers of resistance to infectious diseases.
The purpose of the study was to determine the effect of genes polymorphisms related to disease resistance on the growth and development of pigs and to assess the feasibility of using these markers in selection with the Large White breed. In order to achieve the goal, a number of tasks were solved: typing of SLC11A1 (NRAMP1) and FUT1 genes of the breeding herd of pigs of the inbred type UVB-3 (factory type "Bagachansky"); based on the results of typing, sub-experimental groups were formed and the growth and development of pigs of different genotypes were studied; an assessment of the influence of the specified DNA markers on absolute and relative growth, intensity of formation, tension and uniformity of growth of the experimental stock was carried out; the prospects of selection work based on the investigated DNA markers are determined.
Material and methods. The research was carried out on purebred Large White pigs of the farm type "Bagachansky" of the farm "Plehiv-Agro" of the Poltava region. Genetic studies were carried out at the Institute of Pig Breeding and Agro-Industrial Production of the National Academy of Sciences. DNA typing was performed by the PCR-RFLP method. The live weight of experimental animals was determined monthly starting from weaning at 28 days until reaching the age of six months. Based on the results of weighing, average daily gain, absolute and relative growth, as well as indices of formation intensity, tension and uniformity of growth were calculated. Statistical processing of the obtained data was carried out using the Genalex 6 and Microsoft Excel 2019 programs.
Results and conclusions. It was established that experimental pigs have a sufficiently high level of polymorphism for genetic markers SLC11A1/HinfI 334 C > T and FUT1 SNP g. 307 G > A to carry out an associative analysis. The Polymorphism Information Content index of according to these markers was equal to 0.350 and 0.320 units, respectively, which testifies to the value of the farm type "Bagachanskyi" for preserving the genetic diversity of pigs. It was established that the TT genotype of the DNA marker SLC11A1/HinfI 334 (associated with increased general resistance to infectious diseases) has a positive effect on the intensity of growth of pigs, as evidenced by higher growth after rearing and a 4.86% higher live weight at the age of 6 months (p < 0.05). The AA genotype of the DNA marker FUT1 g.307 G > A (associated with resistance to colibacteriosis) had a positive effect on the uniformity of growth of pigs, at the same time, the growth intensity of the influence of this marker was not detected, which indicates the feasibility of carrying out selection work on this gene to create a line pigs resistant to colibacteriosis. However, among animals with different FUT1 g.307 G > A genotypes, homozygotes with the G allele (susceptible to colibacteriosis) had the highest formation intensity, the difference was 12.19%. Probably, after weaning, piglets susceptible to the disease reduced growth rates after inoculation with opportunistic microorganisms, and after the completion of the infectious process, compensatory growth occurred. This assumption is consistent with the decrease in relative gains in piglets with genotype GG and AG in the period of 28–60 days and the increase in relative gains in these two groups in the period of 2–3 months. Whereas, in the group of piglets with the FUT1 AA genotype, the relative growth constantly decreased with age.
Genotypes associated with better resistance to infectious diseases SLC11A1/HinfI 334 TT and FUT1 AA are characterized by lower intensity of formation and better uniformity of growth, which positively affected the fattening productivity and can be used in selection work. No negative impact of genotypes associated with increased resistance to infectious diseases (and in some cases a positive impact was established) on the growth and development of experimental pigs was found. Marker-associated selection based on the studied genes will contribute to the creation of new structural elements of a Large White breed of pigs with increased growth intensity and improved resistance to infectious diseases.
Bai, C., W. Zhao, and Y. Pan. 2010. Effects of diarrhea and genotype of FUT1 gene on the weight gain during weaning stress in piglets. Journal of Shanghai Jiaotong University-Agricultural Science. 28(5):462–466. URL: https://www.cabdirect.org/cabdirect/abstract/20103370621
Berezovskyy, M. D., O. L. Narizhna, P. A. Vashchenko, A. M. Shostya, S. O. Usenko, L. M. Kuzmenko, and V. H. Slynko. 2021. Terminal boars and other male parents in hybridization system. Bulletin of Poltava State Agrarian Academy. 3:135–141. DOI: https://doi.org/10.31210/visnyk2021.03.16 (in Ukrainian).
Botstein, D., R. L. White, M. Skolnick, and R. W. Davis. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics. 32(3):314–331. https://pubmed.ncbi.nlm.nih.gov/6247908/ (in English).
Devi, B., S. Laskar, P. Borah, I. Hussain, and P. K. Bharti. 2017. Sequencing and phylogenetic analysis of the SLC11A1 gene in pigs. Journal of Applied Animal Research. 45(1):494–497. https://doi.org/10.1080/09712119.2016.1218885 (in English).
Fahmy, M. H., and C. Bernard. 1970. Effect of selection for carcass score on the Genetic Improvement of its components in Swine. Canadian Journal of Animal Science. 50(3):585–592. DOI: https://doi.org/10.4141/cjas70-079 (in English).
Freedeen, H. T. 1958. Selection and Swine Improvement. Animal Breeding Abstracts. 3:229–241 (in English).
Geraci, C., A. R. Varzandi, G. Schiavo, S. Bovo, A. Ribani, V. J. Utzeri, G. Galimberti, L. Buttazzoni, C. Ovilo, M. Gallo, S. Dall'Olio, and L. Fontanesi. 2019. Genetic markers associated with resistance to infectious diseases have no effects on production traits and haematological parameters in Italian large white pigs. Livestock Science. 223:32–38. DOI: http://dx.doi.org/10.1016/j.livsci.2019.03.003 (in English).
Golovko, V. O., R. V. Severin, R. V. Voitenko, V. A. Kochmarski, I. M. Ivanchenko, A. M. Gontar, and M. V. Kuzmenko. 2019. PRRS in the nozoprofile of infectious diseases in pigs in chornuhynsky district of poltava region. Veterinary science, technologies of animal husbandry and nature management. 3:243–249. DOI: http://dx.doi.org/10.31890/vttp.2019.03.33 (in English).
Kay, Z. 2019. 6 most common pig diseases. Retrieved from https://www.wattagnet.com/articles/25841-most-common-pig-diseases-worldwide (in English).
Luc, D. D., N. H. Thinh, H. X. Bo, N. T. Vinh, T. X. Manh, N. V. Hung, and F. Farnir. 2020. Mutation c. 307G > A in FUT1 gene has no effect on production performance of Yorkshire pigs in the tropics: the case of Vietnam. Canadian Journal of Animal Science. 100(3):426–431. http://dx.doi.org/10.1139/cjas-2019-0084 (in English).
Matoušek, V., N. Kernerová, and I. Vrtková. 2011. The variability of chosen genes and their associations with performance traits in sows of přeštice black-pied breed. Research in Pig Breeding. 5(2):13–20 (in English).
Matsenko, O. V., V. M. Mogilyovskyy, O. V. Mitrofanov, Y. V. Maslak, Y. O. Shchepetilnikov, V. A. Pasechnik, and I. V. Furda. 2019. The complex scheme of prevention of gastrointestinal diseases of piglets in farm condition. Veterinary science, technologies of animal husbandry and nature management. 3:144–153. DOI: http://dx.doi.org/10.31890/vttp.2019.03.20 (in English).
Peakall, R., and P. E. Smouse. 2012. GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research – an update. Bioinformatics. 28:2537–2539. http://dx.doi.org/10.1093/bioinformatics/bts460 (in English).
Richards, J. D., J. Gong, and de C. F. M. Lange. 2005. The gastrointestinal microbiota and its role in monogastric nutrition and health with an emphasis on pigs: Current understanding, possible modulations, and new technologies for ecological studies. Canadian Journal of Animal Science, 85(4):421–435. DOI: https://doi.org/10.4141/A05-049 (in English).
Sun, H. S., L. Wang, M. F. Rothschild, and C. K. Tuggle. 1998. Mapping of the natural resistance‐associated macrophage protein 1 (NRAMP1) gene to pig chromosome 15. Animal genetics. 29(2):138–140 (in English).
Tuggle, C. K., L. Marklund, T. J. Stabel, M. A. Mellencamp, and A. Stumbaugh. 2005. Genetic markers for screening animals for improved disease resistance (NRAMP). United States Patent, 6844159B2. http: //ddr.nal.usda.gov/handle/10113/6983 (in English).
Vashchenko, P., A. Saienko, V. Sukhno, O. Tsereniuk, M. Babicz, N. Shkavro, G. Smołucha, and I. Łuszczewska-Sierakowska. 2022. Association of NRAMP1 gene polymorphism with the productive traits of the Ukrainian Large White pig. Medycyna Weterynaryjna. 78(11):563–566. http://dx.doi.org/10.21521/mw.6698 (in English).
Voloshchuk, A. V. 2018. Growth peculiarities of purebred and crossbred pigs with different intensity of formation. Animal Breeding and Genetics. 55:31–38. https://doi.org/10.31073/abg.55.04 (in Ukrainian).
Walsh, P. S., D. A. Metzger, and R. Higuchi. 1991. Chelex 100 as a Medium for Extraction of DNA for PCR-Based Typing from Forensic Material. Bio Techniques. 10:506–509. DOI: http://dx.doi.org/10.2144/000114018 (in English).
Wang, S. J., W. J. Liu, L. G. Yang, C. A. Sargent, H. B. Liu, C. Wang, X. D. Liu, S. H. Zhao, N. A. Affara, A. X. Liang, and S. J. Zhang. 2012. Effects of FUT1 gene mutation on resistance to infectious disease. Molecular biology reports. 39(3):2805–2810. http://dx.doi.org/10.1007/s11033-011-1039-0 (in English).
Xiaoling, D., Z. Xiaodong, Y. Yong, D. Yueyun, X. Weiwei, M. Yun, Z. Weihua, and Y. Zongjun. 2014. Polymorphism, expression of natural resistance-associated macrophage protein 1 encoding gene (NRAMP1) and its association with immune traits in pigs. Asian-Australas Journal of Animal Science. 27:1189–1195. DOI: https://doi.org/10.5713/ajas.2014.14017 (in English).
Ban`kovs`ka, I. B. 2015. Rol` osoby`stosti profesora BV Ban`kovs`kogo v procesi stvorennya ta vdoskonalennya m'yasny`x porid svy`nej (Do 85-richny`ci z dnya narodzhennya) – The role of the personality of Professor BV Bankovsky in the process of creating and improving meat breeds of pigs (To the 85th anniversary of his birth). Pigbreeding. 67:225–235 (in Ukrainian).
Bejdy`k, N. M. 2009. Formuvannya popy`tu na produkciyu organichnogo vy`robny`cztva – Formation of demand for products of organic production. Pigbreeding. 57:50–56 (in Ukrainian).
Berezovs`ky`j, M. D. 2014. Problemni py`tannya z udoskonalennya pleminnogo svy`narstva v Ukrayini ta yix vy`rishennya – Problematic issues of improvement of pedigree pig breeding in Ukraine and their solutions. Pigbreeding. 64:37–48 (in Ukrainian).
Berezovs`ky`j, M. D., and P. A. Vashchenko. 2015. Varianty` poyednan` rizny`x genoty`piv svy`nej v sy`stemi gibry`dy`zaciyi – Variants of combinations of different genotypes of pigs in the hybridization system. Pigbreeding, 67:38–43 (in Ukrainian).
Berezovs`ky`j, M. D., and P. A. Vashchenko. 2019. Selekciya zavods`kogo ty`pu svy`nej u vely`kij bilij porodi – Selection of factory-type pigs in the large white breed. Pigbreeding. Interdepartmental thematic scientific collection of the Institute of Pig Breeding and APV of the National Academy of Sciences. 73:81–90 (in Ukrainian).
Berezovs'kyy, M. D., L. P. Hryshyna, A. A. Hetya, O. A. Man'ko, and P. A. Vashchenko. 2009. Stvorennya vnutriporodnykh zavods'kykh typiv svyney u velykiy biliy porodi z pokrashchenymy m"yasnymy yakostyamy – Creation of intrabreed factory types of pigs in the large white breed with improved meat qualities. Svynarstvo: mizhvidomchyy tematychnyy naukovyy zbirnyk – Pigbreeding: Interdepartmental thematic research collected papers. 57:15–24 (in Ukrainian).
Berezovs`ky`j, M. D., A. O. Ony`shhenko, and P. A. Vashchenko. 2016. Ocinka vidgodivel`ny`x i m'yasny`x yakostej svy`nej vely`koyi biloyi porody` zavods`kogo ty`pu „Bagachans`ky`j” – Evaluation of fattening and meat qualities of pigs of the large white breed of factory type "Bagachanskyi". Pigbreeding. Interdepartmental thematic scientific collection of the Institute of Pig Breeding and APV of the National Academy of Sciences. 68:40–47 (in Ukrainian).
Berezovs`ky`j, M. D., O. L. Nary`zhna, P. A. Vashchenko, and M. M. Odaryuk. 2020. Vidtvoryuval`ni yakosti chy`stoporodny`x i pomisny`x svy`nomatok u poyednanni z terminal`ny`my` knuramy` vlasnogo vidtvorennya ta inshy`my` bat`kivs`ky`my` formamy` – Reproductive qualities of purebred and crossbred sows in combination with terminal boars of their own reproduction and other paternal forms. Pigbreeding. Interdepartmental thematic scientific collection of the Institute of Pig Breeding and APV of the National Academy of Sciences. 74:30–37. DOI: https://doi.org/10.37143/0371-4365-2020-74-03 (in Ukrainian).
Vashchenko, P. A. 2003. Reprodukty`vni yakosti vely`koyi biloyi porody` pry` poyednanni genoty`piv vitchy`znyanoyi i zarubizhnoyi selekciyi – Reproductive qualities of a large white breed when combining genotypes of domestic and foreign breeding. Bulletin of the Poltava State Agrarian Academy. 1–2:165–166. (in Ukrainian).
Vovk, V. O., P. A. Vashchenko, and S. M. Skry`pka. 2012. Vply`v kombinacijnoyi zdatnosti na reprodukty`vni yakosti svy`nej pry` chy`stoporodnomu rozvedenni ta sxreshhuvanni – The influence of combining ability on the reproductive qualities of pigs during purebred breeding and crossing. Pigbreeding. 60:46–49. (in Ukrainian).
Hlazko, V. Y., E. V. Shulha, T. N. Dyman, and H. V. Hlazko. 2001. DNK-tekhnolohii i bioinformatika v reshenii problem byotekhnologii mlekopitaiushchykh – DNA technologies and bioinformatics in solving the problems of mammalian biotechnologies. Belaya Tserkov, BSAU, 487. URL: http://www.irbis-nbuv.gov.ua/cgi-bin/irbis_nbuv/cgiirbis_64.exe (in Russian).
Gorban`, S. 2010. Organichni svy`ni zgidno zi standartamy` – Organic pigs according to standards. Efficient animal husbandry. 6:11–14 (in Ukrainian).
Ivanov, V. O., M. O. Mazan`ko, L. O. Ivanova, and L. V. Zasuxa. 2017. Vy`robny`cztvo i montazh legky`x pry`mishhen` u organichnomu svy`narstvi – Production and installation of light premises in organic pig farming. Bulletin of Sumy NAU: Animal Husbandary. 5 (2):46–50 (in Ukrainian).
Kobernyuk, S. O. 2014. Organichne svy`narstvo v Ukrayini: pereshkody` i napryamy` rozvy`tku – Organic pig farming in Ukraine: obstacles and directions of development. Investments: practice and experience. 13:109–112 (in Ukrainian).
Kovalenko, V. P., S. Yu. Bolelaya, and V. P. Borodaj. 2014. Prognozy`rovany`e plemennoj cennosty` pty`czy po y`ntensy`vnosty` processov rannego ontogeneza – Forecasting the breeding value of birds based on the intensity of early ontogenesis processes. Cytology and genetics. Kyiv, 20(5):360–365 (in Russian).
Kovalenko, T. 2014. Vy`robny`cztvo ogranichnoyi sil`gospprodukciyi i sy`rovy`ny` – Production of foreign agricultural products and raw materials. Agrobusiness today. 5(276):34–42 (in Ukrainian).
Mel`ny`k, Yu., V. Topixa, and A. Volkov. 2002. Nove selekcijne dosyagnennya zavods`ky`j ty`p svy`nej porody` dyurok ukrayins`koyi selekciyi "Stepnoj" – A new breeding achievement of the factory type of pigs of the Durok breed of the Ukrainian selection "Stepnoi". Animal husbandry of Ukraine. 5:17–19 (in Ukrainian).
Ony`shhenko, A. O., O. M. Czsrenyuk, O. V. Akimov, and M. A. Xvatova. 2017. Istoriya, suchasnist` ta napryamy` pokrashhennya produkty`vnosti ukrayins`koyi m'yasnoyi porody` svy`nej – History, modernity and directions for improving productivity of the Ukrainian meat breed of pigs. Pigbreeding, 69:82–90 (in Ukrainian).
Ostapchuk, P. P. 1980. Porody` svy`nej ta yix vy`kory`stannya – Pig breeds and their use. К., Urozhaj, 189 (in Ukrainian).
Pankyeyev, S. P. 2018. Al`ternaty`vni varianty` organichnogo svy`narstva – Alternative options for organic pig farming. Taurian Scientific Bulletin. 100(2):161–167 (in Russian).
Peresadko, L., M. Berezovsky, M. Lutsenko, P. Vashchenko, and S. Manyunenko. 2021. Selekcijna robota iz zavods`ky`m ty`pom «Bagachans`ky`j» u vely`kij bilij porodi svy`nej – The selective work with Bahachansky breed type within the Large White pigs. Animal Husbandry Products Production and Processing. 2:32–40. DOI: https://doi.org/10.33245/2310-9289-2021-166-2-32-40 (in Ukrainian).
Rudoman, H. S., V. M. Balatsky, V. Y. Nor, and V. O. Vovk. 2017. Zviazok polimorfizmu 1849 G > C henu mutsyn 4 iz hospodarsko-korysnymy oznakamy svynei velykoi biloi porody – Association of 1849g > с polymorphism of the mucin 4 gene with economicaly important traits in the Large White pig breed. Scientific and technological bulletin. 117:142–147. doi:10.32900/ntb#nomera (in Ukrainian).
Svechy`n, Yu. K., and L. G. Bary`nova. 1988. Produkty`vnost` svy`nej v zavy`sy`mosty` ot y`ntensy`vnosty` y`x formy`rovany`ya y` ty`pov konsty`tucy`y` – The productivity of pigs depending on the intensity of their formation and types of constitution. Genetics, breeding and selection of pigs: Collection of scientific works of the All-Union Agricultural Institute of Correspondence Education. М., 55–58 (in Russian).
Strizhak, Т. 2015. Do py`tannya po vy`kory`stannyu terminal`ny`x knuriv – As for the issue of terminal boars usage. Bulletin of Agrarian Science of the Black Sea, 2(2):224–227. DOI: 10.31521/2313-092X (in Ukrainian).
Usenko, S. O., M. O. Mazan`ko, A. M. Shostya, O. O. Usenko, V. G. Sly`n`ko, S. V. Chuxlib, and V. I. Berezny`cz`ky`j. 2019. Ekonomichny`j, organizacijny`j ta pravovy`j mexanizm pidtry`mky` i rozvy`tku pidpry`yemny`cztva: kolekty`vna monografiya – Economic, organizational and legal mechanism of support and development of entrepreneurship: collective monograph; edited by O. V. Kalashnyk, H. Z. Mahmudov, I. O. Yasnolob. Astraya Publishing House, 278–285 (in Ukrainian).
Hramkova, O. M. 2020. Gospodars`ko-biologichni osobly`vosti, adaptacijni vlasty`vosti svy`nej irlands`kogo poxodzhennya ta yih vy`kory`stannya za rizny`h metodiv rozvedennya – Economic and biological features, adaptive properties of pigs of Irish origin and their use in different methods of breeding. Doctoral dissertation, Dnipro, 199 (in Ukrainian).
Cerenyuk, O. M., O. V. Akimov, O. I. Chaly`j, and Yu. V. Chereuta. 2017. Kombinacijna zdatnist` za poyednannya poltavs`koyi m'yasnoyi ta uel`s`koyi porid svy`nej – Combining ability for the combination of Poltava meat and Welsh pig breeds. Factors of experimental evolution of organisms. 21:270–273 (in Ukrainian).
Iakubchak, O. M., S. V. Obshtat, and V. M. Mukovoz. 2014. Analiz epizootychnoi sytuatsii infektsiinykh khvorob svynei v Ukraini – Analysis of the epizootic situation of infectious diseases of pigs in Ukraine. Bulletin of Poltava State Agrarian Academy. 3:82–85. DOI: https://doi.org/10.31210/visnyk2014.03.14 (in Ukrainian).
This work is licensed under a Creative Commons Attribution 4.0 International License.