Keywords: meat, beef, halal, storage, research, quality, changes, pH, organoleptic, microbiological indicators


Introduction. In countries of Europe are implementing mechanisms for the humane treatment with farm animals, which provides also minimizing the pain and suffering of animals during slaughter. For this, government of EU in 2009 year adopted the corresponding Regulation № 1099/2009.

Established, that stress before slaughtering animals leads to an increase the level of catecholamines and creatinekinase in their organism, which causes rapid glycolysis, during which accumulates lactic acid in the meat. This leads to a decrease level of glycogen, which causes change pH of the meat and capacity holding the water, the meat acquiring tougher and darker color.

In Ukraine many Ukrainian enterprises of the meat industry received certificates, that attesting the accordance of their products the conditions of "Halal", which opens for them the way to new markets of selling the meat of beef.

However insufficiently studied remains the issue of storing the qualitative indices of halal meat during transportation on long distance. The data about study the date of expiration such meat also absent.

The purpose of this work was to study the qualitative indices of cooled and frozen meat of certified beef of breed of Aberdeen-Angus, to establish the date of expiration.

Research materials and methods. The object of the research: cooled and frozen meat of beef obtained from cattle of breed of Aberdeen-Angus of the company "HALAL MEAT COMPANY" is packed in vacuum bags for establish the date of expiration during storage for 16 months.

In order to conduct the research were taken three samples of halal beef of the Aberdeen-Angus breed: sample № 1 – a neck cut from the scapular part of the carcass, sample № 2 – marbled beef for pork from the scapular part of the carcass, sample № 3 – marbled beef classic from the breast part of the carcass.

The pH of the meat was determined on a pH meter Delta OHM HD2305.0 the Italian company SIMVOLT with a electrode KP70.

Organoleptic evaluation of the product carried out in accordance with DSTU 4589:2006, determination of the number of mesophilic aerobic and facultatively anaerobic microorganisms - in accordance with GOST 10444.15, detection of bacteria of the group of Escherichia coli - in accordance with GOST 21237.

Research on the presence bacteria of L. monocytogenes carried out according to DSTU ISO 11290-1, and the presence bacteria strain of Salmonella carried out in accordance with GOST 21237.

The results processed by methods of variational statistics with using the standard package of programs of Microsoft Excel.

Research results. When measuring the level of pH it established, that in the cooled test samples it was within the normal range and was: sample № 1 – 5.83, sample № 2 – 5.74, sample № 3 – 5.79. When they were stored after thawing the pH decreased. Thus, after 4, 12 and 16 months of storage, the pH of experimental sample № 1 was 5.25; 5.16 and 5.20; sample № 2 – 5.29; 5.21; 5.18; sample № 3 – 5.24; 5.17 and 5.21.

After thawing the test samples, that were stored, occurred loss the moisture. Thus, when they were stored for 4 months the loss of moisture after thawing for sample № 1 was 5.2%, sample № 2 – 4.9%, sample № 3 – 4.3%.

When carrying out organoleptic studies it established, that in all thawed test samples detected signs change of color after 4 months of storage and signs of spoilage after storage of 16 months.

After carrying out microbiological studies it established, that pathogenic microorganisms such as Salmonella and L.monocytogenes detected in samples of thawed meat, which had been stored for 16 months. The number of mesophilic aerobic and facultatively anaerobic microorganisms during storage increased. Thus, in the cooled meat of sample № 1, sample № 2, and sample № 3, this indice was 6.8*103; 6.1*103 and 5.7*103, which did not exceed the requirements of the standard. For thawed test samples after 12 months of storage this indice increases: sample № 1 – 4.6*104, sample № 2 – 3.9*104, sample № 3 – 4.0*104. Exceeding the requirements of the standard of thawed samples of meat noted after 16 months of storage, which leads to their spoilage.

Conclusions. In result of the conducted research it established, that the pH of all experimental samples of cooled meat of beef was within the normal range and was in average 5.78.

During storage of experimental samples after their thawing, the pH decreased, which is associated with damage to fibers of meat caused by the formation crystals of ice during freezing and thawing.

Also, when were stored the experimental samples for 4 months the loss of moisture after defrosting was from 4.3% in sample № 3 to 5.2% in sample № 1.

The change the level of pH in the experimental samples and loss their moisture affected on organoleptic indices: changed the appearance of the meat, color, it has lost the juiciness.

The number of mesophilic aerobic and facultatively anaerobic microorganisms after 16 months of storage exceeded the established standards according to GOST 10444.15.

In the course of research it was established, that the recommended date of storage of halal meat of beef of breed of Aberdeen-Angus is 12 months at a temperature of -18...-25°С in vacuum packs.


Zubchenko, N. I. 2016. Mizhnarodno-pravove spivrobitnytstvo derzhav u sferi zabezpechennya dobrobutu tvaryn ta yikh zakhystu vid zhorstokoho povodzhennya : monohrafiya – International-legal cooperation of states in the sphere of ensuring the welfare of animals and their protection from cruel handling : a monograph. Odesa : Feniks, 284 (in Ukrainian).

Lada, S., G. Harvey Tanakinjal, and H. Amin. 2009. Predicting intention to choose halal products using theory of reasoned action. International Journal of Islamic and Middle Eastern Finance and Management. 2(1):66–76. DOI: 10.1108/17538390910946276 (in English).

Sabow, A. B., A. Q. Sazili, I. Zulkifli, Y. M. Goh, M. Z. A. Ab Kadir, N. R. Abdulla, K. Nakyinsige, U. Kaka, and K. D. Adeyemi. 2015. A comparison of bleeding efficiency, microbiological quality and lipid oxidation in goats subjected to conscious halal slaughter and slaughter following minimal anesthesia. Meat Science. 104:78–84. DOI: (in English).

Authority, E. F. S. 2014. Welfare Aspects of Animal Stunning and Killing Methods. Scientific Report Of The Scientific Panel For Animal Health. EFSAQ-2003-093. 495–507. Italy : Efsa Report. (in English).

Helmut, P. 2010. Review of stunning and halal slaughter. Meat & Livestock Australia. Australia: North Sydney. Locked Bag 991. 26 (in English).

2011. Red Meat Abattoir Association (RMAA). Animal Handling Practical Guidelines for Abattoirs. Available online: Congress 201105.05.2011 (accessed on 18 May 2015). (in English).

2001. Warriss, P. D. 2001. Meat Science: An Introductory Text. 2nd ed. Wallingford, UK: CAB International. 324 (in English).

Aghwan, Z. A., A. U. Bello, A. A. Abubakar, J. C. Imlan, and A. Q. Sazili. 2016. Efficient halal bleeding, animal handling, and welfare: a holistic approach for meat quality. Meat Science, 121, 420–428 (in English). DOI:

Ali, S. A. M., H. O. Abdalla, and I. M. Mahgoub. 2011. Effect of slaughtering method on the keeping quality of broiler chickens' meat. Egypt Poultry Science, 31(4):727–736 (in English).

Jiang, J., L. Zhang, J. Yao, Y. Cheng, Z. Chen, and G. Zhao. 2022. Effect of a static magnetic field assisted thawing on the physicochemical quality and microstructure of frozen beef tenderloin. Frontiers in Nutrition, 9:1–12. DOI: (in English).

Dalvi, M., N. Hamdami, and A. Le-Bail. 2016. Effect of freezing under electrostatic field on the quality of lamb meat. Innovative Food Science & Emerging Technologies, 37:68–73. DOI: (in English).

2007. DSTU 4589:2006 Napivfabrykaty miasni naturalni vid kompleksnoho dilennia yalovychyny za kulinarnym pryznachenniam. Tekhnichni umovy. 12 (in Ukrainian).

2010. GOST 10444.15-94. Produkty pishevye. Metody opredeleniya kolichestva mezofilnyh aerobnyh i fakulta-tivno-anaerobnyh mikroorganizmov.7 (in Russian).

2006. GOST 21237-75. Myaso. Metody bakteriologicheskogo analiza. 27 (in Russian).

2017. DIN EN ISO 11290-1:2017 (ISO 11290-1:1996) Mikrobiolohiia kharchovykh produktiv ta kormiv dlia tvaryn. Horyzontalnyi metod vyiavlennia ta pidrakhunku Listeria monocytogenes. Chastyna 1. Metod vyiavlennia. 49 (in Ukrainian).

Kolisnyk, O. I. 2009. Khimichnyi sklad miasa aberdyn-anguskykh bychkiv riznykh henotypiv. Visnyk Poltavskoi derzhavnoi ahrarnoi akademi, 153–155 (in Ukrainian).

Hamoen, J. R., H. M. Vollebregt, and R. G. М. Van der Sman. 2013. Prediction of the time evolution of pH in meat. Food Chemistry. 141(3):2363–2372 (in English). DOI: j.foodchem.2013.04.127. Hwang I. H., Thompson J. M. The interaction between pH and temperature decline early postmortem on the calpain system and objective tenderness in electrically stimulated beef longissimus dorsi muscle. Meat Science. 2001. V. 58 (2). P. 167–174. DOI: (in English).

Hwang, I. H., and J. M. Thompson. 2001. The interaction between pH and temperature decline early postmortem on the calpain system and objective tenderness in electrically stimulated beef longissimus dorsi muscle. Meat Science. 58 (2):167–174). DOI: (in English).

Guignot, F., C. Touraille, A. Ouali, M. Renerre, and G. Monin. 1994. Relationships between post-mortem pH changes and some traits of sensory quality in veal. Meat Science. 37(3):315–325. DOI: (in English).

Cheng, L., D.-W. Sun, Z. Zhu, and Z. Zhang. 2015. Emerging techniques for assisting and accelerating food freezing processes: a review of recent research progresses. Critical Reviews in Food Science and Nutrition, 57(4):769–781. DOI: 1004569 (in English).

Markovych, I. I. 2015. Zminy mikrobiolohichnykh pokaznykiv napivkopchenykh kovbas u protsesi zberihannia. Naukovi dopovidi Natsionalnoho universytetu bioresursiv i pryrodokorystuvannia Ukrainy, 3(52):18–24 (in Ukrainian).

You, Y., T. Kang, and S. Jun. 2020. Control of ice nucleation for subzero food preservation. Food Engineering Reviews, 13:15–35. DOI: (in English).

Okuda, K., A. Kawauchi, and K. Yomogida. 2020. Quality improvements to mackerel (Scomber japonicus) muscle tissue frozen using a rapid freezer with the weak oscillating magnetic fields. Cryobiology, 95(10):130–137. DOI: j.cryobiol.2020.05.005 (in English).

Dell’Orto, V., G. Savoini, C. A. Sgoifo Rossi, S. Vandoni, R. Compiani, А. Agazzi, and M. Ferroni. 2010. Valutazione oggettiva strumentale del colore delle carcasse di vitello al macello: Quaderni della Ricerca. Regione Lombardia: Università degli studi di Milano. 51 (in English).

How to Cite
Peshuk, L. V., Simonova, I. I., Vdovychenko, Y. V., Dzhus, P. P., & Dedova, L. O. (2022). ANALYSIS OF QUALITY INDICATORS OF CHILLED AND FROZEN BEEF. Animal Breeding and Genetics, 64, 47-55.
Breeding and Selection

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