SPERM PRODUCTIVITY AND FERTILIZATION CAPACITY OF SPERMATOZOA OF HOLSTEIN STUD BULLS IN CONDITIONS OF LLC “UKRAINIAN GENETIC COMPANY”

Keywords: stud bulls, Holstein breed, parameters of sperm productivity, fertilization capacity of spermatozoa, sperm productivity index, correlation

Abstract

Effective selection and breeding in dairy farming is impossible without artificial insemination, results of artificial insemination largely depend on the quality of semen of bulls-improvers. Objective. To ascertain parameters of sperm productivity and fertilization capacity of spermatozoa of Holstein stud bulls of foreign selection in conditions of LLC “Ukrainian Genetic Company”. Methods. We have analyzed ejaculates of 20 mature Holstein stud bulls elite-record class brought to animal breeding enterprise from Germany and the Netherlands. The duration of use of bulls at the breeding enterprise is on average 4–5 years.Quantitative indicators of sperm were taken per year to avoid the influence of seasonal factors. Semen was collected twice a week by artificial vagina on the false stud. Ejaculates obtained were analyzed according to the DSTU 35.35-97 incertified production laboratory of LLC “Ukrainian Genetic Company”, equipped with up-to-date equipment for sperm quality assessment, packing, freezing and storage. Quantity and quality characteristics of sperm were analyzed on computer sperm analyzer IVOS (HamiltonThorneResearch, USA). Sperm fit for cryopreservation is diluted at a temperature of +35°С in the environment AndroMed (Germany) at rate no less than 20 million spermatozoids for one sperm dose and packed into 0.25 ml straws. Frozen sperm doses after check are kept in the special bio-storage in liquid nitrogen at a temperature of -196°С.

Results. Fertilization capacity ofthe spermatozoaof experimental stud bulls was measured by the percentage of quantity of cows and female calves that were impregnated after the first insemination in Zhytomyr (PAF “Ierchyky”, SPDG “Nova Peremoga”, ALLC “Ptakhoplemzavod “Korobivskyi”) and Kyiv (LLC “Agrofirm “Kyivska”) regions. On the whole results of insemination of 12525 cows and 1230 female calves of puberty age were analyzed.

Sperm productivity index and average fertilization capacity of spermatozoa of stud bulls was assessed by the method of M. M. Maiboroda, S. H. Hermanchuk, Yu. P. Polupan, and D. M. Basovsʼkyy.

It was revealedsignificant variability of sperm productivity indicators of experimental stud bulls, which vary within: number of obtained high-quality ejaculates within one year –32–173 pcs., total volume of native sperm – 201–1016 ml, average value of spermatozoa concentration in 1 ml – 1.51–3.52 milliard, average sperm motility in ejaculate – 7.2–83 points. Bull studs of LLC “Ukrainian Genetic Company” are characterized by a rather high index of sperm productivity which equals from 5.19 to 15.29 billion of motile spermatozoids in ejaculate.

Average fertilization capacity of spermatozoa of experimental Holstein bulls in conditions of 4 farms of Zhytomyr and Kyiv region ranges from 37.1 to 61.4%, average figure for examined livestock is 50.9%. During the research we found that stud bulls whose sperm fertilization capacity was above 50% in most cases (6 out of 8) had index of sperm productivity above average – 9.86–15.25billion of motile spermatozoids in ejaculate.

We have detected positive and statistically significant correlation between fertilization capacity of spermatozoa and the average value of concentration, motility of spermatozoids in ejaculate (+0.474 and+0.417 respectively) and sperm productivity index (+0.639). Conclusion. Our research proves dependence of fertilization capacity of sperm on its quality parameters. The results we obtained suggest that it is expedient to use index of sperm productivity to assess quality of sperm and prove its importance for breedingbulls selection.

References

Havrylenko, M. S. 2008. Vplyv hodivli ta utrymannya na vidtvoryuval'nu funktsiyu molochnykh koriv˗ Influence of feeding and keeping on the reproductive function of dairy cows. Naukovo – tekhnichnyy byulleten' – Scientific and technical bulletin. 96:90–93 (in Ukrainian).

Vyshnevs'kyy, L. V., S. L. Voytenko, and O. V. Sydorenko. 2018. Monitorynh produktyvnosti velykoyi rohatoyi khudoby molochnykh porid v pleminnykh stadakh doslidnykh hospodarstv NAAN ta rekomendatsiyi shchodo yiyi pokrashchennya – Monitoring of dairy cattle productivity in breeding herds of NAAS experimental far ms and recommendations for its improvement. Poltava : PP «Astraya», 24 (in Ukrainian).

Kyzebnyy, S. V., and O. V. Boiko. 2018. Otrymannya, otsinka, zberihannya i vykorystannya spermy plidnykiv sil's'kohospodars'kykh tvaryn – Obtaining, evaluating, storing and using sperm of sires of farm animals. In M.V.Hladii & Yu. P. Polupan (Eds.). Selektsiyni, henetychni ta biotekhnolohichni metody udoskonalennya i zberezhennya henofondu porid sil's'kohospodars'kykh tvaryn – Breeding, genetic and biotechnological methods of improvement and preservation of the gene pool of farm animals. Poltava, LLC ”Techservice Company”, 709–720 (in Ukrainian).

Maiboroda, M. M., S. H. Hermanchuk, Yu. P. Polupan, and D. M. Basovsʼkyy. 2019. Metodyka rozrakhunku pleminnoyi tsinnosti buhayiv, koriv ta molodnyaku i vidboru yikh za selektsiynymy indeksamy – Methods of calculation the breeding value of Bulls, Cows and Young Animals of the Cattle and selecting them by selectoin indices. Chubynske : Institute of Animal Breeding and Genetics nd. a. M.V.Zubets NAAS, 20 (in Ukrainian).

Pryshed'ko, V. M. 2014. Ekonomichna efektyvnist' pleminnoho vykorystannya buhayiv-plidnykiv riznykh typiv stresostiykosti – Economic efficiency of the pedigree use oxen inseminators of different types of stress resistance. Naukovyy visnyk LNUVMBT im. Gzhyts'koho – Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. 2(59):169–175 (in Ukrainian).

Siratskyi, Y. Z., Ye. I. Fedorovych, V. V. Fedorovych, V. O. Kadysh, and L. M. Piddubna. 2008. Fiziolohobiokhimichni ta biotekhnolohichni pocaznyky spermy buhaiv-plidnykiv – Physiological, biochemical and biotechnological parameters of sperm of breeding bulls. Kyiv : Liuksar, 208 (in Ukrainian).

Uhnivenko, A. M., L. A. Koropets', S. Yu. Demchuk, D. K. Nosevych. 2017. Naukovi zasady vidtvoryuvannya poholiv"ya velykoyi rohatoyi khudoby m"yasnykh porid: monohrafiya - Scientific principles of reproduction of beef cattle: a monograph. Kyyiv, TsP KOMPRYNT, 400 (in Ukrainian).

David, I., P. Kohnke, G. Lagriffoul, O. Praud, F. Plouarboué, P. Degond, and X. Druart. 2015. Mass sperm motility is associated with fertility in sheep. Anim Reprod Sci, 161:75–81 (in English).

DOI: https://doi.org/10.1016/j.anireprosci.2015.08.006 49 (in English).

Flowers, W. L. 2013. Triennial Reproduction Symposium: sperm characteristics that limit success of fertilization. J Anim Sci., 91(7):3022–9 (in English). DOI: https://doi.org/10.2527/jas.2012-5945 (in English).

Gopinathan, A., S. N. Sivaselvam, S. K. Karthickeyan, K. Kulasekar, J. J. Kirubaharan. and R. Venkataramanan. 2018. Effect of Non-genetic factors on Semen Quality Traits of Crossbred Holstein Friesian Bulls (Bos taurus x Bos indicus) in Organized Farming Conditions at Tamil Nadu, India. International Journal of Current Microbiology and Applied Sciences, 7(11):3219–3229 (in English). DOI: https://doi.org/10.20546/ijcmas.2018.711.370 (in English).

Hering, D. M., K. Olenski, and S. Kaminski. 2014. Genome-wide association study for poor sperm motility in Holstein-Friesian bulls. Anim Reprod Sci, 146:89–97 (in English). DOI: https://doi.org/10.1016/j. anireprosci.2014.01.012

Hossain, D. M., M. Talukder, M. K. Begum, and A. K. Paul. 2016. Determination of Factors that Affect the Pregnancy Rate of Cows after Artificial Insemination at MonirampurUpa.zila of Jessore District of Bangladesh. Journal of animal reproduction and biotechnology, 31(4):349 353 (in English). DOI: https://doi.org/10.12750/jet.2016.31.4.349 (in English).

Islam, M., A. Apu, S. Hoque, M. Ali, and S. Karmaker. 2018.Comparative study on the libido, semen quality and fertility of Brahman cross, Holstein Friesian cross and Red Chittagong breeding bulls. Bangladesh Journal of Animal Science, 47(2):61–67 (in English). DOI: https://doi.org/10.3329/bjas.v47i2.40236 (in English).

Hamid, J., and L. Alemayehu. 2015. Review on Major Factors Affecting the Successful Conception Rates on Biotechnological Application (AI) in Cattle Review on Major Factors Affecting the Successful Conception Rates on Biotechnological Application (AI) in Cattle. Global Journal of Medical Research: G Veterinary Science and Veterinary Medicine, 15:19–27 (in English).

Morrell, JM, T. Nongbua, S. Valeanu, I. Lima Verde, K. Lundstedt-Enkel, A. Edman, and A. Johannisson. 2017.Sperm quality variables as indicators of bull fertility may be breed dependent. Anim Reprod Sci., 185:42–52 (in English). DOI: https://doi.org/10.1016/j.anireprosci.2017.08.001.

Murphy, E. M., A. K. Kelly, C. O’Meara, B. Eivers, P. Lonergan, and S. Fair. 2018. Influence of bull age, ejaculate number, and season of collection on semen production and sperm motility parameters in Holstein Friesian bulls in a commercial artificial insemination centre. Journal of Animal Science, 96(6):2408–2418 (in English). DOI: https://doi.org/10.1093/jas/sky130 (in English).

Nagy, S, A. Johannisson, T. Wahlsten, R. Ijäs, M. Andersson, and H. Rodriguez-Martinez. 2013. Sperm chromatin structure and sperm morphology: their association with fertility in AI-dairy Ayrshire sires. Theriogenology, 79:1153–1161 (in English). DOI: https://doi.org/10.1016/j.theriogenology.2013.02.011 (in English).

Rabidas, S. K., A. K. Talukder, Md. G. S. Alam, and F. Y. Bari. 2012. Relationship between Semen Quality Parameters and Field Fertility of Bulls. J. Emb. Trans., 27:21–28 (in English).

Tanga, B. M., A. Y. Qamar, S. Raza, S. Bang, X. Fang, K. Yoon and J. Cho. 2021. Semen evaluation: methodological advancements in sperm quality-specific fertility assessment - A review. Anim Biosci. 34(8):1253–1270 (in English). DOI: https://doi.org/10.5713/ab.21.0072 (in English).

Zăhan, M, E. Pall, M. Cenariu, I. Miclea, and A. Dascăl. 2018. Relationship between in vitro semen parameters and bull fertility. ABAH Bioflux, 10(2):156–163 (in English).

Published
2021-12-08
How to Cite
Zakharchuk, D. (2021). SPERM PRODUCTIVITY AND FERTILIZATION CAPACITY OF SPERMATOZOA OF HOLSTEIN STUD BULLS IN CONDITIONS OF LLC “UKRAINIAN GENETIC COMPANY”. Animal Breeding and Genetics, 62, 136-144. https://doi.org/10.31073/abg.62.18