CONDITION AND PROSPECTS INITIATED BY PROFESSOR V. YU. NEDAVA RESEARCH IN NANOBIOTECHNOLOGY

Keywords: ultrafine silica, nanomaterial, spermatozoa, oocytes, in vitro cultivation, embryos, concentration, cryopreservation

Abstract

Doctor of Agricultural Sciences, Professor Volodymyr Yukhymovich Nedava (1925–2009) made a significant contribution to the development of selection, genetics and biotechnology in animal husbandry in Ukraine. His efforts laid the foundation for biotechnological foundations of farm animal reproduction and modern breed theory, substantiated the use of heterosis in livestock, effective methods of improving the brown Carpathian breed, simplified the method of assessing breeding animals for feed production.

Goal. To analyze the directions of scientific activity of Doctor of Agricultural Sciences, Professor V. Yu. Nedava in the context of development of research in nanobiotechnology and to generalize its main achievements in developing the theory and methodology of application of ultra fine silica in media for cryopreservation of sperm farm animals.

Methods. General scientific (analytical-synthetic, logical, systemic), biographical and source methods. The source base of the research covers a wide range of materials, which are based on archival documents and primary sources, scientific works (publications), reports and memoirs of colleagues about the talented scientist.

Results. At the initiative and direct participation of V. Yu. Nedava, a method was developed for the treatment of bull semen, which differed from the conventional one in that in order to stabilize the biological value, to the freshly obtained sperm before dilution is added UFS at the rate of 3–30 mg per 1 billion sperm.

Later, Professor V. Yu. Nedava and co-authors published the results of research on the use of fine pyrogenic UFS (Aerosil) as a stabilizer of the biological value of bull sperm, which was confirmed by higher fertility rates of heifers after insemination with Aerosil sperms (5.8% higher compared to control).

Continuation of the initiated research of V. Yu. Nedava on the use of ultra fine silica to stabilize the cell surface of reproductive gametes was research on improving the technology of in vitro embryo formation and cryopreservation of male epididymal sperm using nanomaterials, which began in 2007.

Conclusions. A number of scientific works of Professor Volodymyr Yukhymovich Nedava testify to his talented organizational and scientific character traits, which have a continuation in our time. V. Yu. Nedava initiated the development of the concept of VDK processing of semen of farm animals, determined their purpose in the system of conservation of genetic resources of farm animals, substantiated the importance of using such materials as the main link in the conservation of farm animals.

References

Alyushin, M. T., and M. N. Pogorelyj. 1971. Ajerosil i ego primenenie v farmacevticheskoj praktike – Aerosil and its use in pharmaceutical practice. Farmaciya – Pharmacy. 6:73–77 (in Russian).

Belous, A. M., V. I. Gryshhenko, and Yu. S. Parashhuk. 1986. Kriokonservaciya reproduktivnіkh kletok – Cryopreservation of reproductive cages. AN USSR, Instytut problem kriobiologiyi i kriomedycyny – Academy of Sciences of the Ukrainian SSR, Institute of Problems of Cryobiology and Cryomedicine. K.: Naukova Dumka. 207 (in Russian).

Bojko, Yu. M., I. S. Boroday, and O. V. Shcherbak. 2012. Istoriya Instytutu rozvedennya i genetyky tvaryn u podiyax, faktakh, biografiyakh uchenykh – History of the Institute of Animal Breeding and Genetics in the events, facts, biographies of scientists. Luxar. 368 (in Ukrainian).

Burkat, V. P., and S. I. Kovtun. 2008. Suchasna biotekhnolohiya v tvarynnytstvi– Modern biotechnology in animal husbandry. Biotekhnolohiya – Biotechnology. 1(3):7–12 (in Ukrainian).

Burkat, V. P., and I. S. Borodaj. 2008. Narysy z istoriyi instytutu: monografiya – Essays from the history of the institute: monograph. Kyyiv, Agrarna nauka, 556 (in Ukrainian).

Galagan, N. P. 2006. Nanomateryaly na osnove vysokodyspersnogo kremnezema i biomolekul v sredakh s reproduktivnymi kletkami – Nanomaterials Based on Fine Silica and Biomolecules in Media with Reproductive Cells. Materialy nauch. konf. Sorbenty kak faktory kachestva zhyzni i zdorovya – Sorbents as factors of quality of life and health: materials of II All-Russian. scientific conf. Moskva-Belgorod. 55–59 (in Russian).

Zyuzyun, A. B., O. V. Shherbak, S. I. Kovtun, A. O. Svergunov, and G. O. Svergunova. 2019. Analiz efektyvnosti rozvytku poza organizmom embrioniv svynej za vykorystannya nanobiomaterialu – Analysis of the efficiency of development of porcine embryos outside the body using nanobiomaterial. Faktory eksperymentalnoyi evolyuciyi organizmiv: Zb. nauk. pr. Nacionalna akademiya nauk Ukrayiny – Factors of experimental evolution of organisms: coll. Science. National Academy of Sciences of Ukraine. К., Logos. 25:231–236. DOI: https://doi.org/10.7124/FEEO.v25.1168 (in Ukrainian).

Zyuzyun, A. B., O. V. Shherbak, and O. S. Osypchuk. 2015. Zastosuvannya nanomaterialu v embriogenetychnij systemi in vitro otrymannya embrioniv svynej – The use of nanomaterial in the embryogenic system in vitro of pig embryos. Faktory eksperymentalnoyi evolyuciyi organizmiv: Zb. nauk. pr. Nacionalna akademiya nauk Ukrayiny – Factors of experimental evolution of organisms: coll. Science. National Academy of Sciences of Ukraine. Kyiv, Logos. 17:164–168 (in Ukrainian).

Kovtun, S. I., O. V. Shherbak, and A. B. Zyuzyun. 2012. Vykorystannya nanomaterialiv dlya efektyvnogo formuvannya embrioniv svynej in vitro – The use of nanomaterials for the efficient formation of pig embryos in vitro. Dosyagnennya i problemy genetyky, selekciyi ta biotexnologiyi– Achievements and problems of genetics, selection and biotechnology. 4:513–518 (in Ukrainian).

Kovtun, S. I., A. B. Zyuzyun, O. V. Shherbak, and P. A. Troczkyj. 2018. Vykorystannya nanobiotexnologichnykh metodiv dlya optymizaciyi texnologiyi kultyvuvannya oocytiv koriv poza organizmom – The use of nanobiotechnological methods for optimizing the technology of culturing oocytes of cows outside in vitro. Faktory eksperymentalnoyi evolyuciyi organizmiv: Zb. nauk. pr. Nacionalna akademiya nauk Ukrayiny – Factors of experimental evolution of organisms: coll. Science. National Academy of Sciences of Ukraine. К., Logos. 22:257–261 (in Ukrainian).

Kovtun, S. I., O. V. Shherbak, and P. A. Troczkyj. 2013. Zastosuvanny nanomaterialiv u systemi zberezhennya bioriznomanittya silskogospodarskykh tvaryn Ukrayiny – Application of nanomaterials in the system of biodiversity conservation of agricultural animals of Ukraine. Faktory eksperymentalnoyi evolyuciyi organizmiv: Zb. nauk. pr. Nacionalna akademiya nauk Ukrayiny – Factors of experimental evolution of organisms: coll. Science. National Academy of Sciences of Ukraine. К., Logos. 13:57–61 (in Ukrainian).

Kovtun, S. I., N. P. Galagan, and O. V. Shherbak. 2016. Ocinka biologichnoyi aktyvnosti nanobiomaterialiv – Assessment of biological activity of nanobiomaterials. Rozvedennya i genetyka tvaryn – Animal breeding and genetics. 52:186–193 (in Ukrainian).

Kurbatov, A. D., E. M. Platov, N. V. Korban, L. G. Moroz, and V. A. Nauk. 1988. Kriokonservacija spermy sel'skohozjajstvennyh zhivotnyh – Cryopreservation semen of farm animals. L.: Agropromizdat. 256 (in Russian).

Nedava, V. E., A. A. Chujko, L. A. Begma, A. A. Begma, and V. I. Bogomaz. 1990. Ispolzovaniye ajerosilov v praktyke isskustvennogo osemenenyya – The use of aerosil in the practice of artificial insemination. Zootehnija – Zootechnia. 8:63–65 (in Russian).

Nedava, V. E., O. I. Smyrnova, and M. P. Zhuravel. 1992. Ob ispolzovanyyi vysokodyspersnykh kremnozemov v sredax dlya zamorazhyvanyya spermy baranov – On the use of finely dispersed silicas in media for freezing sheep sperm. Sel'skohozjajstvennaja biologija – Agricultural biology. 4:220–225 (in Russian).

Novychkova, D. A., T. Y. Kuzmyna, O. V. Shherbak, N. P. Galagan, and O. A. Epyshko. 2017. Vlyyaniye nanochastyc vysokodyspersnogo kremnezema na morfologyyu i intracytoplazmatycheskuyu lokalyzacyyu lypydnykh kapel v oocytakh svynej – The effect of fine silica nanoparticles on the morphology and intracytoplasmic localization of lipid droplets in pig oocytes. Rozvedennya i henetyka tvaryn – Animal breeding and Genetics. 53:284–292 (in Russian).

Chujko, A. A., V. E. Nedava, V. K. Pikalov, L. A. Begma, V. I. Bogomaz, A. A. Begma, and V. M. Ogenko. Patent № 1144698 SSSR. MKI A 61 D 7/02. Sposob obrabotki spermy sel'skohozjajstvennyh zhivotnyh – Patent No. 1144698 of the USSR. MKI A 61 D 7/02. Method for processing sperm of farm animals. Zajavl. 03.06.1983; opubl. 15.03.85, Byul – Claim 06.03.1983; publ. 03.15.85, Byul. 10:5 (in Russian).

Galagan, N. P., A. P. Sinel'nik, V. E. Nedava, M. P. Zhuravel', O. I. Smirnova, A. A. Chuyko, V. I. Bogomaz, V. K. Pikalov, E. A. Bakay, N. V. Kovalenko, and L. F. Lukomskaya. Patent № 1727816 A 1 SSSR. MKI A 61 D 19/02. Sreda dlya razbavleniya spermy baranov – Patent No. 1727816 A 1 of the USSR. MKI A 61 D 19/02. Sheep semen dilution medium. Zayavl. 18.07.88; opubl. 23.04.92, Byul – Claim 07.18.88; publ. 04.23.92, Byul. 15:3 (in Russian).

Chujko, A. A., V. K. Pogorelyj, A. A. Pentyuk, M. A. Andrejchyn, and L. A. Belyakova. 2003. Medecynskaya chymyya i klynycheskoye primenenyye dyoksyda kremniya – Medical chemistry and clinical use of silicon dioxide. Kiev, Naukova dumka. 415 (in Russian).

Shherbak, O. V., S. I. Kovtun, and A. B. Zyuzyun. 2015. Biotexnologichna model oderzhannya zarodkiv kroliv in vitro z vykorystannyam nanomaterialu – Biotechnological model of rabbit embryo production in vitro using nanomaterial. Biologiya tvaryn – Animal biology. 17(2):172–178 (in Ukrainian).

Shherbak, O. V., A. B. Zyuzyun, O. S. Osypchuk, S. I. Kovtun, N. P. Galagan, and P. A. Troczkyj. 2017. Vyvchennya biologichnoyi aktyvnosti nanomaterialu v umovakh kultyvuvannya spermatozoyidiv ta oocytiv svynej in vitro – The study of the biological activity of nanomaterial under the conditions of cultivation of pig sperm and oocytes in vitro. Faktory eksperymentalnoyi evolyuciyi organizmiv: Zb. nauk. pr. Nacionalna akademiya nauk Ukrayiny – Factors of experimental evolution of organisms: coll. Science. National Academy of Sciences of Ukraine. Kyiv: Logos. 20:256–260 (in Ukrainian).

Shherbak, O. V., and L. M. Goncharenko. 2011. Vplyv nanomaterialiv na zhyttyezdatnist kriokonservovanykh spermatozoyidiv bugayiv – Influence of nanomaterials on the viability of cryopreserved bull sperm. Visn. Stepu : nauk. zb. «Agropromyslove vyrobnycztvo Ukrayiny – stan ta perspektyvy rozvytku» – Bulletin of the Steppe: Science. zb. Kirovograd: Tsentr.-Ukr. ed., 2011. – Anniversary. issue : Materials VII All-Ukrainian. scientific-practical conf. of young scientists and specialists "Agro-industrial production of Ukraine – state and prospects of development", March 24. 145–147 (in Ukrainian).

Shherbak, O. V. 2014. Efektyvnist zastosovuvannya nanomaterialiv u texnologiyi racionalnogo vykorystannya genetychnykh resursiv svynej – Nanomaterials efficiency in pig genetic resource management technology. Rozvedennya i henetyka tvaryn – Animal breeding and genetics. 48:277–284 (in Ukrainian).

Shherbak, O. V., N. P. Galagan, P. A. Troczkyj, and S. I. Kovtun. 2017. Zastosuvannya nanochastynok dioksydu kremniyu v texnologiyi formuvannya embrioniv svynej in vitro – The use of silica nanoparticles in the formation of pig embryos in vitro. Zbirnyk naukovykh pracz «Nanosystemy, nanomaterialy, nanotexnologiyi» – Nanosystems, nanomaterials, nanotechnologies: coll. Science. wash. 15(2):381–388 (in Ukrainian).

Kovtun, S., O. Shcherbak, and S. Shekhovtsov. 2017. Analysis of the impact of nanomaterials on the viability of sperm. Scientific conference with international participation Animal Science. Challenges and innovations Proceedings Institute of Animal Science. 410–413 (in English).

Kovtun, S. I., N. P. Galagan, O. V. Shcherbak, N. Y. Klymenko, and O. S. Osypchuk. 2013. Nanobiotechnologies in the System of Farm Animalsʼ Gene Pool Preservation. Nanocomposites, Nanophotonics, Nanobiotechnology, and Applications. Selected Proceedings of the Second FP7 Conference and International Summer School Nanotechnology: from fundamental research to innovations. 156:215–221. DOI: https://doi.org/10.1007/978-3-319-06611-0_18 (in English).

Kovtun, S. I., N. P. Galagan, O. V. Shcherbak, O. S. Osypchuk, and N. Y. Klymenko. 2015. Nanocomposites in Technology of Cryopreservation of Sperm Boar. Nanoplasmonics, Nano-Optics, Nanocomposites, and Surface Studies. Selected Proceedings of the Second FP7 Conference and the Third International Summer School Nanotechnology: From Fundamental Research to Innovations. 167:387–394. DOI: https://doi.org/10.1007/978-3-319-18543-9_26 (in English).

Shcherbak, O. V., A. B. Zyuzyun, A. O. Sverhunov, and A. O. Sverhunova. 2019. Features of the influence of UFS/Raffinose nanocomposites on in vitro cultivation of gamets of swine. Nanophotonics, Nanooptics, Nanobiotechnology, and Their Applications. NANO 2018. 222:255–263. DOI: https://doi.org/10.1007/978-3-030-17755-3_17 (in English).

Published
2020-05-04
How to Cite
Kovtun, S. I., Shcherbak, O. V., Lyuta, I. M., & Shevchenko, T. V. (2020). CONDITION AND PROSPECTS INITIATED BY PROFESSOR V. YU. NEDAVA RESEARCH IN NANOBIOTECHNOLOGY. Animal Breeding and Genetics, 59, 124-135. https://doi.org/10.31073/abg.59.14