Keywords: colibacteriosis, Escherichia coli, Ukrainian meat breed, pigs, FUT1 gene, MUC4 gene


Each year about 10 million piglets die all over the world because of oodema disease and postweaning diarrhea. These diseases associated with the adhesion of enterotoxic Escherichia coli on the surface of pig intestinal cells are called colibacteriosis. The disease exciter is enteropathogenic hemolytic strains of Eschenchia (E. coli). The enterotoxic E. coli O157 is able to produce specific adhesins in piglet colibacteriosis, the most important role being played by F18 and F4 (K 88). The genetic resistance of piglets to diarrhea is based on the lack of appropriative receptors on the surface of the intestinal cells of animals. It is practical interest in pigs breading to study using polymorphism of E. coli F18 and F4 (ECR F18/FUT1 and ECR F4/MUC4) receptor genes in the selection process associated with the occurrence of colibacteriosis in piglets. The studies were apropiate on the basis of a breeder reproducer of the Ukrainian meat breed pigs of Dnipropetrovsk state agricultural institute selection at "Lugovskoye" Ltd. of firm "Avias 2000" of Solonyansky district, Dnipropetrovsk region. Microbiological studies of the presence of E. coli O157 in the animals faeces were performed at the Department of Biotechnology of the Ukrainian Chemical Technology University, using a ready-made selective Compact Dry EC medium. Animal genotype estimation for FUT1 and MUC4 genes was carried out at the genetic laboratory of the Institute of Pig Breeding andAgro Industrial Production, National Academy of Agrarian Sciences of Ukraine, by the PCR-RFLP method. Studies have been conducted to detect enterotoxigenic E. coli O157 in a closed population of pigs of Ukrainian meat breed type of selection of Dnepropetrovsk state agricultural institute. 58.3% of clinically healthy sows were found to contain E. coli O157, while in the 5–12 days group of clinically healthy pigs the detection rate was 20.0% and in piglets with signs of diarrhea 44.5%. On 12th day the average weight of sick piglets was lower on 30.45% compared to the average weight of healthy individuals was found, and the losses among sick pigs at weaning on day 21 reached to 56%. The relationship between colibacteriosis piglets susceptibility with genotypes of FUT1 and MUC4 genes was identified. The studied population of pigs is unreliable for colibacteriosis, so a gene pool study for the presence of the desired colibacteriosis resistance genotypes of FUT1 and MUC4 marker genes, followed by selection of parental cross-breeding forms, should be included in the livestock reproduction program.


Bolockij, I. A., A. K. Vasil'ev, V. I. Semencov, and S. V. Prucakov. 2007. Infekcionnye bolezni svinej – Swine infection diseases. Rostov-na-Donu, Feniks, 346 (in Russian).

Dvorkin, G. L., and А. А. Gutkovskiy. 1989. Kolibakterioz telyat I porosyat. Faktory virulentnosti vozbuditelya, epizootologiya, diagnostika, mery bor'by – Colibacteriosis of calves and piglets. Pathogen virulence factors, epizootology, diagnosis, control measures. Minsk, 70 (in Russian).

Limarenko, A. A. 2008. Bolezni svinej. Spravochnik. – Diseases of pigs. Directory. St.-Petersburg, Lan’, 640 (in Russian).

Sobko, A. I., V. F. Romanenko, G. K. Bozhko, V. D. Nastenko, E. G. Pavlov, A. I. Zavirjuha, T. V. Pashov, V. A. Bortnichuk, N. F. Jashhenko, N. M. Lapshin, V. S. Shehovcov, K. P. Korzh, V. E. Chumachenko, L. P. Okuncov, N. F. Jashhenko, P. S. Derevjanko, I. P. Derevjanko, S. I. Nozdrachev, I. N. Gladenko, O. A. Malinin, V. D. Shuljak, L. I. Pogrebnjak, and Z. I. Pilipec. 1988. Spravochnik po boleznjam svinej – Pig Disease Handbook. Kiev, Urozhaj, 360 (in Russian).

Wilson, R. A., and D. H. Francis. 1986. Fimbriae and enterotoxins associated with Escherichia coli serogroups isolated from pigs with colibacillosis. Am. J. Vet. Res. 47:213–217 (in English).

Herman, L., N. Botteldoorn, and S. De Smet. 2002. Relation between colibacillosis Escherichia coli F18 and a polymorphism in the alphafucosyltransferase gene. Second international symposium on Candidate Genes for Animal Health (C. G. A. H). Montpellier, France, August 16-18th 2002 : abstracts. 1 (in English).

Jeyasingham, M. D., P. Butty, T. P. King, R. Begbie, and D. Kelly. 1999. Escherichia coli K88 receptor expression in intestine of disease-susceptible weaned pigs. Vet. Microbiol. 68:219–234 (in English).

Joller, D., H. U. Bertschinger, E. Bürgi, C. B. Jorgensen, M. Malek, H. Jörg, S. Genini, and Р. Vögeli. 2006. Linkage mapping of the porcine Mucin 4 gene on SSC13 and association with the Escherichia coli F4ac receptor gene. Pig Genome I. An Overview of Cutting–edge Genomics with Emphasis on the Pig. Parco Tecnologico Padano: 52 (in English).

Joller, D., C. B. Jorgensen, H. U. Bertschinger, P. Python, I. Edfors, S. Cirera, A. L. Archibald, E. Bürgi, P. Karlskov–Mortensen, L. Andersson, M. Fredholm, and P. Vögeli. 2009. Refined localization of the Escherichia coli F4ab/F4ac receptor locus on pig chromosome 13. Animal Genetics. 40(5):749–52. doi: 10.1111/j.1365-2052.2009.01881.x (in English).

Joller, D., C. B. Jorgensen, H. U. Bertschinger, P. Python, I. Edfors, S. Cirera, A. L. Archibald, E. Bürgi, P. Karlskov–Mortensen, L. Andersson, and M. Fredholm. 2006. Refined linkage mapping of the Escherichia coli F4ac receptor gene on pig chromosome 13. Proceeding of the 30th International Conference of Animal genetic. Brazil: abstracts. 59 (in English).

Performance Tested SM : Certificate No. 110402. The AOAC Research Institute hereby certifies that the performance of the test kit known as: Compact Dry EC manufactured by NISSUI Pharmaceutical Co., Ltd. Ueno, Taitoku Tokyo 1108736 Japan (in English).

Horwitz, W. 2000. Official Method of Analisis of AOAC. International, 17th ed. AOAC International. Gaithersburg, Maryland, 2200 (in English).

DSTU ISO 4832. 2018. Mіkrobіologіya harchovih produktіv ta kormіv dlya tvarin. Gorizontal'nij metod pіdrahuvannya kolіform. Metod pіdrahuvannya kolonіj – Microbiology of food and animal feed. Horizontal method of counting coliforms. The method of counting colonies. ISO 4832:2006 (in Ukrainian).

DSTU ISO 16649-2. 2016. Mіkrobіologіya harchovih produktіv і kormіv dlya tvarin. Gorizontal'nij metod pіdrahuvannya β-glyukuronіdaza-pozitivnih Escherichia coli. Castina 2. Tekhnіka pіdrahuvannya kolonіj za teperaturi 44◦C z vikoristannyam 5-bromo-4-hloro-3-іndolіl-ß-D-glyukuronіdu – Microbiology of food and animal feed. Horizontal method of counting β-glucuronidase-positive Escherichia coli. Part 2: Technique for counting colonies at a temperature of 44ºC using 5-bromo-4-chloro-3-indolyl-β-D-glucuronide. ISO 16649-2:2001 (in Ukrainian).

Dubreuil, J. D., R. E. Isaacson, and D. M. Schifferli. 2016. Animal enterotoxigenic Escherichia coli. EcoSalPlus. doi: 10.1128/ecosalplus.ESP-0006-2016 (in English).

Saenko, A. M., V. M. Balatskij, G. І. Syrovnev, and V. T. Smetanіn. 2012. Polіmorfіzm lokusіv FUT1 ta MUC4 u populyaciyi svinej ukrains'koy myasnoy porodi selekciyi Dnіpropetrovskogo SGІ – Polymorphism of FUT1 and MUC4 loci in population of Ukrainian meat breed pigs selected at Dnepropetrovsk agricultural Institute. Svinarstvo – Pig breeding. 60:76–79 (in Ukrainian).

Boswortth, В. Т., and P. Vögeli. 2003. Patent US 6596923 A1. Methods and compositions to identify swine genetically resistant to F18 E. coli associated diseases. Publ. date: Jul. 22 (in English).

Instrukcіya z bonіtuvannya svinej; Іnstrukcіya z vedennya plemіnnogo oblіku u svinarstvі – Instructions for bonding pigs. Instructions from the tribal region in pigs. 2003. Kyyiv, Minagropolіtiki Ukrainy. 64 (in Ukranian).

Yan, X. M., J. Ren, Y. M. Guo, N. S. Dings, K. F. Chen, J. Gao, H. S. Ai, C. Y. Chen, J. W. Ma, and L. S. Huang. 2003. Research on the genetic variations of alfa–1–fucosytransferase (FUT1) gene in 26 pig breeds. Yi Chuan Xue Bao. 30(9):830–834 (in English).

Vögeli, P., H. U. Bertschinger, M. Stamm, C. Stricker, C. Hagger, R. Fries, J. Rapacz, and G. Stranzinger. 1996. Genes specifying receptors for F18 fimbriated Escherichia coli, causing oedema disease and postweaning diarrhea in pigs, map to chromosome 6. Anim. Genet. 27:321–328 (in English).

Rampoldi, A., M. J. Jacobsen, H. U. Bertschinger, D. Joller, E. Bürgi, P. Vögeli, L. Andersson, A. L. Archibald, M. Fredholm, C. B. Jørgensen, and S. Neuenschwander. 2011. The receptor locus for Escherichia coli F4ab/F4ac in the pig maps distal to the MUC4–LMLN region. Mamm Genome. 22(1–2):122–129 (in English).

Vijtiuk, N., V. Bilić, I. Harapin, I. Vrbanac, and I. Valpotić. 2001. Differentiated attaching and effacing activities of porcine F4+ enterotoxigenic and nonenterotoxigenic Escherichia coli strains. Period. Biol. 102:21–27 (in English).

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