INFLUENCE OF NANOPARTICLES OF HIGHLY DISPERSED SILICA ON MORPHOLOGY AND INTRACYTOPLASMIC LOCALIZATION OF LIPID DROPLETS IN PORCINE OOCYTES

  • D. A. Novichkova Federal State Budget Scientific Institution "All-Russian Research Institute of Genetics and Breeding of Agricultural Animals" (St. Petersburg – Pushkin, Russian Federation)
  • T. I. Kuzmina Federal State Budget Scientific Institution "All-Russian Research Institute of Genetics and Breeding of Agricultural Animals" (St. Petersburg – Pushkin, Russian Federation)
  • O. V. Shcherbak Institute of Animal Breeding and Genetics nd. a. M.V.Zubets of NAAS (Chubynske, Ukraine)
  • N. P. Galagan Institute of Surface Chemistry. A. A. Chuyko National Academy of Sciences of Ukraine (Kiev, Ukraine)
  • O. A. Epishko UO "Grodno State Agrarian University" (Grodno, Republic of Belarus)
Keywords: porcine oocytes, lipid droplets, Nile red, BCB- test, highly dispersed silica

Abstract

Based on the visualization by the fluorescent probe (Nile red) of intracellular lipids in porcine oocytes that have finished growth phase in vivo or in vitro morphology and distribution of lipid drops in oocytes before and after cultivation with nanoparticles of highly dispersed silica (0.001% of HDS) have been characterized. In the cultivation of oocytes with HDS the level of oocytes that have finished growth phase in vitro with lipid droplets in the form of granules and diffuse type of distribution increases in comparison with the above-indicated markers in the oocytes of the other studied groups. The results of the experiments make it possible to interpret the obtained data on the form of lipids in the form of granules, as a form that determines the high potencies of oocytes for further development and assume that the transformation of granules into clusters during cultivation is considered as a predictor of subsequent destructive changes in the oocyte.

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Published
2017-04-27
How to Cite
Novichkova, D. A., Kuzmina, T. I., Shcherbak, O. V., Galagan, N. P., & Epishko, O. A. (2017). INFLUENCE OF NANOPARTICLES OF HIGHLY DISPERSED SILICA ON MORPHOLOGY AND INTRACYTOPLASMIC LOCALIZATION OF LIPID DROPLETS IN PORCINE OOCYTES. Animal Breeding and Genetics, 53, 284-292. https://doi.org/10.31073/abg.53.40